Identifcation of the mecA gene by PCR and PCR-RT of Staphylococcus aureus
DOI:
https://doi.org/10.36790/epistemus.v13i26.97Keywords:
Staphylococcus aureus, MRSA, PCR, PCR-RTAbstract
At present, the application of more efficient techniques that contribute to the identification of pathogens such as Staphylococcus aureus more quickly has become essential because these pathogens are part of the main problem in nosocomial infections. The persistence of the problem is due to the fact that these pathogens have developed resistance to environmental threats. The excessive use of sanitizers and antibiotics such as methicillin are the cause of the resistance that in this
case comes from the mecA gene. The identification of this gene is crucial to differentiate resistant and non-resistant strains. However, taking into count that traditional processes for the identification of this pathogen are usually very slow, techniques such as PCR are applied because of their efficacy and efficiency. However, techniques such as PCR-RT have improvements that provide some advantage compared to its predecessor.
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Figueroa , E.M. and M.D. Navarrete Prevalencia de staphylococcus aureus resistente a meticilina y caracterización del factor de virulencia leucocidina panton-valentine asociado a factores de riesgos en estudiantes de la carrera de medicina de la Universidad de las Américas. 2019, Quito: Universidad de las Américas, 2019.
Wu, S., et al., Prevalence and Characterization of Food-Related Methicillin-Resistant Staphylococcus aureus (MRSA) in China.Frontiers in Microbiology, 2019. 10(304). DOI: https://doi.org/10.3389/fmicb.2019.00304
Peralta Quito and S. Gabriela, Factores asociados a infecciones por Staphylococcus aureus resistente a la meticilina, Hospital Vicente Corral Moscoso. Cuenca, 2016-2018. 2019.
Armas Fernández, A., et al., Resistencia de Staphylococcus aureus a la meticilina en aislamientos nosocomiales en un hospital provincial. Gaceta Médica Espirituana, 2015. 17: p. 80-91.
Reischl, U., et al., Rapid Identification of Methicillin-ResistantStaphylococcus aureus and Simultaneous Species Confirmation Using Real-Time Fluorescence PCR. Journal of Clinical Microbiology, 2000. 38(6): p. 2429-2433. DOI: https://doi.org/10.1128/JCM.38.6.2429-2433.2000
Tamay de Dios, L., C. Ibarra, and C. Velasquillo, Fundamentos de la reacción en cadena de la polimerasa (PCR) y de la PCR en tiempo real. Investigación en discapacidad, 2013. 2(2): p. 70-78.
Santander, H.C., et al., ADN polimerasas bacterianas. Archivos de medicina, 2018. 14(2): p. 4.
Lázaro, R. and E.L. Leandro, Implementación del método de detección de adenovirus en moluscos bivalvos mediante la técnica de reacción en cadena de la polimerasa (PCR). 2017.
Pedrosa Amado, A., Reacción en cadena de la polimerasa.Revista Archivo Médico de Camagüey, 1999. 3: p. 0-0.
Pahissa Berga, A., Identificación, in Infecciones producidas por Staphylococcus aureus, m. ICG, Editor. 2009: Valencia, españa. p. 22.
Varela-M, R.E., J.S. Arias, and L.E. Velásquez, Estandarización de una prueba múltiple de reacción en cadena de la polimerasa en tiempo real para la identificación de Angiostrongylus cantonensis, A. costaricensis y A. vasorum. Biomédica, 2018. 38: p. 111-119. DOI: https://doi.org/10.7705/biomedica.v38i0.3407
Poma, H.R., et al., Comparación de la eficiencia de extracción de ácidos nucleicos utilizando distintos kits comerciales y empleando la qPCR. Efecto de las sustancias inhibidoras.Revista Argentina de Microbiología, 2012. 44(3): p. 144-149.
Fusco, V., G. Blaiotta, and K. Becker, Staphylococcal Food Poisoning, in Food Safety and Preservation. 2018, Elsevier. p. 353-390. DOI: https://doi.org/10.1016/B978-0-12-814956-0.00012-3
Costa, A.-M., I. Kay, and S. Palladino, Rapid detection of mecA and nuc genes in staphylococci by real-time multiplex polymerase chain reaction. Diagnostic microbiology and infectious disease, 2005. 51(1): p. 13-17. DOI: https://doi.org/10.1016/j.diagmicrobio.2004.08.014
ULLOA, M.T., et al., Comparación de reacción de polimerasa en cadena, látex y antibiograma para detección de Staphylococcus aureus meticilina resistente. Revista chilena de infectología, 2001. 18: p. 255-260. DOI: https://doi.org/10.4067/S0716-10182001000400003
Brigitte, T. and C. Chávez, Virus del papiloma humano identificados por biología molecular. Hospital Andino. Riobamba. mayo 2017–junio 2018. 2018, Universidad Nacional de Chimborazo, 2018.
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